TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ ▼S; however, the amino acid in the P1’ position can also be G, A, M, C, or H (1). It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins. TEV Protease has a 7xHis-tag for easy removal from a reaction using nickel affinity resins and has been engineered to improve thermal stability and decrease autolysis.
Product Source
Cloned from Tobacco Etch Virus and expressed in E. coli. - This product is related to the following categories:
- NEBExpress MBP Fusion and Purification System,
- Nickel Purification (His-tag),
- Bacterial E. coli Protein Expression,
- Proteases Products,
- Protein Purification,
Protein Expression
- This product can be used in the following applications:
- Fusion Protein Cleavage,
- Target Protein Insolubility ,
- Protein Purification,
- Protein Digestion,
Protein Expression