Trypsin-ultra™, Mass Spectrometry Grade is a serine endopeptidase. It selectivelycleaves peptide bonds C-terminal to lysineand arginine residues (1). Trypsin-ultra istreated with L-(tosylamido-2-phenyl) ethylchloromethyl ketone (TPCK) to inactivate anyremaining chymotryptic activity. It is modifiedby acetylation of the ε-amino groups of lysineresidues to prevent autolysis. Trypsin-ultra (TPCKtreated)cleaves at Lys-Pro and Arg-Pro bondsat a much slower rate than other amino acidresidues (2).
Product Source
Isolated from bovine (Bos taurus) pancreas.
Reconstitution
Trypsin-ultra, Mass Spectrometry Grade should be reconstituted by the addition of 20–200 μl of high purity water. Rapid autolysis is a function of enzyme concentration.
This product is related to the following categories:
Proteases Products,
Proteome Analysis Products
This product can be used in the following applications: