SARS-CoV-2 E (QHD43418.1, 1 a.a. - 75 a.a.) recombinant protein with an initial Met, His-Avi tag at N-terminal expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
11.4
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purification:
Affinity purification
Purity:
> 95% by SDS-PAGE.
Endotoxin Level:
< 0.1 EU/ug of the protein by LAL method.
Activity:
Measured by its binding ability in a functional ELISA. Immobilized E (SARS-CoV-2) Recombinant Protein at 2 ug/mL (100 uL/well) can bind Recombinant 2019-nCoV Nucleocapsid with a linear range of 1.2-41.1 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie BlueThis protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 12 kDa.
Storage Buffer:
In 20 mM Tris, 250 mM NaCl, pH 8.0 (0.5% TritonX-100).
Storage Instruction:
Store at -80°C.Aliquot to avoid repeated freezing and thawing.