SARS-CoV-2 N (QHD43423.2) full-length recombinant protein with His tag at C-terminal expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
47.08
Specificity:
No non-specific binding to normal human serum was identified so far.
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purification:
Ni column
Purity:
> 95% by SDS-PAGE.
Quality Control Testing:
DTT-reducing SDS-PAGE Result
Recommend Usage:
AntigenELISAWestern BlotThe optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris, pH 8.5.
Storage Instruction:
This protein is not stable at 4°C in liquid. For long term storage store at -20 to -80°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Applications
Western Blot
Antigen
Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated by N protein, 100 uL/well, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hour at 37°C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2 M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.
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