The activity is determined by the ability to chemoattract THP-1 cells and is detectable starting at 10 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie BlueLane 1: non-reducing conditionsLane 2: reducing conditions
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere.After reconstitution with sterilized water, store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Human THP-1 cells were allowed to migrate to rat Ccl5 at (0, 0.1, 1, 10, 100 and 1000 ng/mL). After 45 minutes, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above.