产品概述:产品参数 外观:橙红色液体 Abs (pH 3)= 464 nm (before photolysis) Abs/Em (after photocrosslinking to nucleic acid)= 510/610 nm 分子量:511.5 储存条件 -20℃避光保存,有效期见外包装。 产品介绍 PMA 是一种高亲和性的 DNA 结合染料,该染料本身具有微弱的荧光,但与核酸结合后可以发出更为明亮的荧光。它尤其与双链 DNA 具有高亲和性。PMA 不能透过细胞膜,因此只能选择性标记死细胞上暴露在外的 DNA。这个特性使得 PMA 可以通过实时定量 PCR(qPCR)的手段,广泛用于可被培养的病原细胞的筛选,因为 PMA 可与死细胞上的 DNA 牢固结合,而不能用于 PCR 反应的扩增。PMA 的这个特性使其可以通过实时定量 PCR 的手段,广泛用于可被培养的病原细菌的筛选。注意事项 1. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。 2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
说明书:UE-P4051 使用本产品的文献:参考文献1.Detection and quantification of viable Bacillus cereus group species in milk by propidium monoazide quantitative real-time PCR应用方向:Bacillus cereus(蜡样芽孢杆菌)2.Persistence of Bacteroides ovatus under simulated sunlight irradiation应用方向:Bacteroides ovales(卵巢杆菌)3.Advantageous Direct Quantification of Viable Closely Related Probiotics inPetitSuisse Cheeses under In Vitro Gastrointestinal Conditions by Propidium Monoazide - qPCR应用方向:Bifidobacterium animalis(动物双歧杆菌)4.Assessing the diagnostic importance of nonviable bacterial cells in respiratory infections应用方向:Burkholderia cepacia(赤胸鲨)5.Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions应用方向:Campylobacter jejuni(空肠弯曲菌)6.Viability-PCR Shows That NAAT Detects a High Proportion of DNA from Non-Viable Chlamydia trachomatis应用方向:Chlamydia trachomatis(沙眼衣原体 )7.Digestibility and prebiotic properties of potato rhamnogalacturonan I polysaccharide and its galactose-rich oligosaccharides/oligomers应用方向:Clostridium leptum(瘦肉梭菌)8.Survival and persistence of host-associated Bacteroidales cells and DNA in comparison with Escherichia coli and Enterococcus in freshwater sediments as quantified by PMA-qPCR and qPCR应用方向:Enterrococcus spp(肠球菌属)9.Detection of viable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR应用方向:Escherichia coli(大肠杆菌)10.Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR应用方向:Helicobacter pylori(幽门螺杆菌)11.Probiotic bacteria survive in Cheddar cheese and modify populations of other lactic acid bacteria应用方向:Lactobacillus acidophilus(嗜酸乳杆菌)12.Fate of Viable but Nonculturable Listeria monocytogenes in Pig Manure Microcosms应用方向:Listeria monocytogenes(单球菌)13.A multicenter study of viable PCR using propidium monoazide to detect Legionella in water samples应用方向:Legionella pneumophila(嗜肺军团菌)14.Propidium monoazide and Xpert MTB/RIF to quantify Mycobacterium tuberculosis cells应用方向:Mycobacterium tuberculosis(结核分枝杆菌)15.Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods应用方向:Nitrosomonas europae(欧洲硝酸铵)16.Study and Understanding Behavior of Alginate-Inulin Synbiotics Beads for Protection and Delivery of AntimicrobialProducing Probiotics in Colonic Simulated Conditions应用方向:Pediococcus acidilactici(酸乳球菌)17.Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR应用方向:Pseudomonas aeruginosa(铜绿假单胞菌)18.Multiplex PCR coupled with propidium monoazide for the detection of viable Cronobacter sakazakii, Bacillus cereus, and Salmonella spp. in milk and milk products应用方向:Salmonella enterica(肠炎沙门菌)19.Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide-Modified Quantitative PCR应用方向:Staphylococcus aureus(金黄色葡萄球菌)20.Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide应用方向:Streptococcus oralis(口腔链球菌)21.OxyRactivated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis应用方向:Vibrio cholerae(霍乱弧菌)22.A New Physiological Role for the DNA Molecule as a Protector against Drying Stress in Desiccation-Tolerant Microorganisms应用方向:Microbacterium 3J1(微杆菌3J1)