Formulation:Recombinantproteinstoredin50mMTris-HCl,pH7.5,150mMNaCl,10mMglutathione,0.1mMEDTA,0.25mMDTT,0.1mMPMSF,25%glycerol.
StorageandstABIlity:Storeproductat-70ºC.Stableuptooneyear.
ScientificBackground:PI4KBisamemberofthephosphatidylinositol4-kinasefamily(PI4K)andisthefirstcommittedstepintheproductionofthesecondmessengerinositol-1,4,5-trisphosphate(PIP3).PI4KBregulatesthegolgidisintegration/reorganizationduringmitosispossIBLyviathephosphorylationofphosphatidylinositol.PI4KBisatypeIIIenzymethatissensitivetowortmanninandisresponsibleforregulatingthesynthesisofagoNIST-sensitivepoolsofpolyphosphoinositides.PI4KBiscriticalforglucose-inducedinsulinsecretionduetoitscapacitytoregulatethereleaseofsecretorygranules.PI4KBinhibitstheinsulin-stimulatedtranslocationofglucosetransporter-4inmouseADIpocytesthroughitsinteractionwithneuronalcalciumsensor-1.
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