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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | PAK1 (Phospho-Ser199) Antibody |
| Synonyms | Serine/threonine-protein kinase PAK 1; p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK; PAK1 |
| Immunogen | The antiserum was produced against synthesized phospho-peptide derived from human PAK1 around the phosphorylation site of Ser199. |
| Immunogen Range | |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
| Specificity | PAK1 (Phospho-Ser199) Antibody detects endogenous levels of PAK1 only when phosphorylated at Ser199. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | PAK1 |
| Database Links |
Gene ID: 5058 Swiss-Prot #: Q13153 OMIM #: 602590 Unigene #: Hs.435714 |
| Target Modification | Phospho |
| Modification Sites | H:S199, M:S199, R:S198 |
| Application Images | |
| Image 1 |
 Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PAK1 (Phospho-Ser199) Antibody |
| Image 2 |
 Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAK1 (Phospho-Ser199) Antibody. |
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