The antiserum was produced against synthesized phospho-peptide derived from human ABL1 around the phosphorylation site of Thr735.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
ABL1 (Phospho-Thr735) Antibody detects endogenous levels of ABL1 only when phosphorylated at Thr735.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ABL1
Database Links
Gene ID: 25 Swiss-Prot #: P00519
OMIM #: 189980/608232 Unigene #: Hs.431048
Target Modification
Phospho
Modification Sites
H:T735, M:T734, R:T753
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ABL1 (Phospho-Thr735) Antibody
Image 2
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using ABL1 (Phospho-Thr735) Antibody.