Endo D, also known asEndoglycosidase D, is a recombinant glycosidase,which cleaves within the chitobiose core ofpaucimannose N-linked glycans, with or withoutextensions in the antennae. Endo Dis tagged with a chitin binding domain (CBD) foreasy removal from a reaction, and is suppliedglycerol-free for optimal performance in HPLC andMS- intensive methods.
Product Source
A truncated Endo D gene cloned from Streptococcus pneumoniae and expressed in E. coli as a fusion to chitin binding domain
Specificity
Detailed SpecificityFigure 1: Detailed specificity of Endo DThereaction contained 0.08 mU of α1-2 Mannosidase (Prozyme#GKX-5009), 50 units of Endo D and 2 μl 0.5M Na0Ac pH 5.0 buffer in a total reaction volume of 10 μl.Reactions were incubated at 37°C for 2 hours. Followingremoval of the bottom branch, Endo D is activeon a complex upper arm (1).
This product is related to the following categories:
Endoglycosidases Products,
Proteome Analysis Products
This product can be used in the following applications: