Substrate Specificity:α1-6 Mannosidase is a highly specific exoglycosidase that removes unbranched α1-6 linked D-mannopyranosyl residues from oligosaccharides (1,2). When used in conjunction with α1-2,3 Mannosidase, the α1-6 Mannosidase will cleave α1-6 Mannose residues from branched carbohydrate substrates.Figure 1:Detailed specificity of α1-6 MannosidaseAll reactions contained 32 units of α1-2,3 Mannosidase (NEB #P0729 ), 40 units of α1-6 Mannosidase, 1X GlycoBuffer 1 and 1X BSA In a total reaction volume of 10 µl. Reactions were incubated at 37°C. The substrate depicted in (E) will not cut to completion. If this structure exists in any substrate it will be impervious to cleavage by α1-6 Mannosidase. Note: When used alone, α1-6 Mannosidase will still act only on linear substrates. When used in conjunction with α1-2,3 Mannosidase, the α1-6 Mannosidase will cleave α1-6 Mannose residues from branched carbohydrate substrates.
Product Source
Cloned from Xanthomonas manihotis and expressed in E. coli.
This product is related to the following categories:
Exoglycosidases Products
This product can be used in the following applications:
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