Description:
NeuraminidaseisthecommonnameforAcetyl-neuraminylhydrolase(Sialidase).α2-3,6,8,9NeuraminidaseAcatalyzesthehydrolysisofalllinearandbranchednon-reducingterminalsialicacidresiduesfromglycoproteinsandoligosaccharides.Theenzymereleasesα2-3andα2-6linkagesataslightlyhigherratethanα2-8andα2-9linkages.
Specificity
DetailedSpecificity
α2-3,6,8,9NeuraminidaseAwillcleavebranchedsialicacidresiduesthatarelinkedtoaninternalresidue.Thisoligosaccharidefromfetuinisanexampleofaside-branchsialicacidresiduethatcanefficientlybecleaved(1).
ProductSource
ClonedfromArthrobacterureafaciensandexpressedinE.coli(2).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
GlycoBuffer1 | -20 | 10X |
Notes:
Reactionsmaybescaled-uplinearlytoaccommodatelargerreactionvolumes.Theamountofexoglycosidaseenzymerequiredvarieswhendifferentsubstratesareused.Startwith1–2μlfor1μgofglycoproteinor100nMofoligosaccharideforonehourina10–25μlreaction.Ifthereisstillundigestedmaterial,letthereactiongoovernight.Higherconcentrationsofenzymeaswellaslongerincubationtimesmaybenecessaryforcleavageofbranchedstructures.