PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F digestion deaminates the aspargine residue to aspartic acid, and leaves both the protein and the oligosaccharide intact, keeping them suitable for further analysis (1).Developed for proteomic applications, Rapid PNGase F (non-reducing format) is a reformulated version of Rapid PNGase F (NEB #P0710 ) that allows the complete and rapid deglycosylation of antibodies and fusion proteins in minutes, while preserving disulfide bonds. All N-glycans are released rapidly and without bias, facilitating high throughput proteomics applications and methods for antibody characterization by mass spectrometry such as intact mass analysis. Rapid PNGase F (non-reducing format) combines the advantages of Rapid PNGase F (fast processing time), with the non-reducing conditions preserving quaternary structure.ESI-TOF analysis of an antibody before and after treatment with Rapid PNGase F (non-reducing format).
Specificity
Rapid PNGase F (non-reducing format) cleaves all complex, hybrid and high-mannose type glycans from antibodies and related proteins. Core α1,3 fucosylation (found in immunoglobulins expressed in plant or insect cells) is resistant to both Rapid PNGase F and PNGase F.
This product is related to the following categories:
Endoglycosidases Products,
Proteome Analysis Products
This product can be used in the following applications:
购物车为空，快去购物吧！
4000-520-616
咨询顾问
特别 提示
