Description:
Peptide:N-GlycosidaseF,alsoknownasPNGaseF,isarecombinantamidasewhichissuppliedglycerolfreeforoptimalperformanceinHPLCintensivemethods.PNGaseFcleavesbetweentheinnermostGlcNAcandasparagineresiduesofhighmannose,hybrid,andcomplexoligosaccharidesfromN-linkedglycoproteins(1).
ProductSource
ClonedfromElizabethkingiamiricola(formerlyFlavobacteriummeningosepticum)andexpressedinE.coli(2).Specificity
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
GlycoproteinDenaturingBuffer | -20 | 10X |
GlycoBuffer2 | -20 | 10X |
NP-40 | -20 | 10% |
Notes:
Todeglycosylateanativeglycoprotein,longerincubationtimeaswellasmoreenzymemayberequired.SincePNGaseF(Glycerol-free),RecombinantactivityisinhibitedbySDS,itisessentialtohaveNP-40inthereactionmixture.Itisnotknownwhythisnon-ionicdetergentcounteractstheSDSinhibitionatthepresenttime.PNGaseF(Glycerol-free),Recombinant willnotcleaveN-linkedglycanscontainingcoreα1-3Fucose.Recommendedstoragetemperatureis4°C,avoidrepeatfreeze-thawcycles.