Description:
PNGaseAisarecombinantamidase,whichcleavesbetweentheinnermostGlcNAcandasparagineresiduesofhighmannose,hybrid,andshortcomplexoligosaccharidessuchasthosefoundinplantandinsectcellsfromN-linkedglycoproteinsandglycopeptides.PNGaseAdiffersfromPNGaseFinthatitcleavesN-linkedglycanswithorwithoutα(1,3)-linkedcorefucoseresidues.
SubstrateSpecificity
PNGaseAhydrolyzesN-glycanchainsfromglycoproteins/peptidesregardlessofthepresenceofxyloseorfucose.[x=HorManorGlcNAc].ProductSource
ClonedfromOryzasativa(rice)andexpressedinPichiapastoris(1).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
GlycoBuffer3 | -20 | 10X |
GlycoproteinDenaturingBuffer | -20 | 10X |
NP-40 | -20 | 10% |
Notes:
PNGaseAisactiveonbothglycoproteinsandglycopeptides.PNGaseAcannotcleavelargerN-glycanssuchasthosefromFetuin,Fibrinogen,IgG,LactoferrinandTransferrin.PNGaseAisabletocleavehighmannoseN-glycanstructuresfromMan3uptoMan9.Reactionsmaybescaled-uplinearlytoaccommodatelargerreactionvolumes.