Description:
Peptide-N-GlycosidaseF,alsoknownasPNGaseF,isanamidasethatcleavesbetweentheinnermostGlcNAcandasparagineresiduesofhighmannose,hybrid,andcomplexoligosaccharidesfromN-linkedglycoproteins(1)
ProductSource
PNGaseFispurifiedfromFlavobacteriummeningosepticum(3)anditisfreeofproteasesandEndoFactivities.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
GlycoproteinDenaturingBuffer | -20 | 10X |
GlycoBuffer2 | -20 | 10X |
NP-40 | -20 | 10% |
Notes:
SincePNGaseFactivityisinhibitedbySDS,itisessentialtohaveNP-40presentinthereactionmixture.Whythisnon-ionicdetergentcounteractstheSDSinhibitionisunknownatpresent.Todeglycosylateanativeglycoprotein,longerincubationtimeaswellasmoreenzymemayberequired.PNGaseFwillnotcleave N-linkedglycanscontainingcoreα1-3Fucose.Typicalreactionconditions:PleaseseeProtocols