当前位置 ： Millipore >>> Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells
Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells
  • Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

Millipore/NS830 | GFAP ELISA Kit/NS830/1 kit96 wells

价格: ¥6288.00 市场价: 10480.00
打印产品信息
货号: NS830
品牌: Millipore
规格
数量
库存(0)
特别 提示
代购产品：无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
立即购买 加入购物车

收藏商品
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616

  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • Description
      CatalogueNumberNS830
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionGFAPELISAKit
      OverviewBackground
      GlialFibrillaryAcidicProtein(GFAP)isaclass-IIIintermediatefilamentuniquelyandubiquitouslyexpressedaspartofthestructuralnetworkinastrocyticglialcells.ThisspecificexpressionmakesGFAPidealasabroadastrocyticMarkerinvertebrates.Astrocytesplayavarietyofkeyrolesinsupporting,guiding,nurturing,andsignalingneuronalarchitectureandactivityinahighlyinterdependentmanner.CurrentunderstandingofCNSstructuresuggeststhatthereareatleastasmanyglialcellsastheestimated100billionneurons,butperhapsupwardsof100timesmore.Thus,
      thehealthofbothneuronsandastrocytesisoftenaffectedindiseaseandtrauma.GFAPisprovingtobeausefulbiomarkerinthedetectionofneuraldegeneration.AlthoughdefectsinGFAPhavebeenreportedtocausecertainrareconditions,suchasAlexander’sdisease(Brenneretal.,2001),theobservedinterconnectedphysiologyofneuronalandglialcellshasenabledtheuseofGFAPasaneurodegenerationdetectiongauge.Thisrelationshipisbeingexploitedbyresearchersandcliniciansmeasuringavarietyofneurologicalissuesfromcancertotrauma.GFAPhasbeenusedasabiomarkerintheearlydetectionofchemicallyinducedcancer(Capoetal.,1997)andclassicalglioblastomas(Jungetal.,2007).ElevatedlevelsofGFAPhavebeenreportedintraumaticbraininjury(TBI)andappeartodirectlycorrelatewiththeseverityofinjuryandbraintissueoxygenationincombatcasualtycareresearch(CCR,2008)aswellascivilianaccidents(Mondelloetal.,2010).GFAPhasalsobeenidentifiedasagoodbiomarkerinstudiesoftoxininducedacutebraininjury(Liaoetal.,2008),indifferentiationofhemorrhagicvs.ischemicstrokes(Foerchetal.,2006),aswellasincardiacarrest(Kanekoetal.,2009).MilliporenowoffersanELISAassayforsensitivemeasurementofGFAPproteinintissuehomogenatesandBIOLOGicalfluids(serum,plasma,CSF,andserumfreemedia)fromhuman,mouse,andrat.

      PrincipalsofProcedure
      ThisassayisaSandwichELISAbasedsequentiallyon:1)captureofsolubleGFAPproteintothewellsofamicrotiterplatecoatedbyapre-titeredamountofanti-GFAPmonoclonalantibody;2)removalofunboundmaterialbywashing;3)bindingofbiotinylatedanti-GFAPmonoclonalantibodyto
      thepreviouslycapturedGFAPprotein;4)removalofunboundmaterialbywashing;5)bindingofstreptavidinconjugatedhorserADIshperoxidasetothebiotinylatedantibodies;6)removalofexcessorunboundenzymeconjugatesbywashing;and7)quantificationofimmobilizedantibody-enzyme
      complexesbymonitoringhorseradishperoxidaseactivityinthepresenceofthesubstrate3,3’,5,5’-tetramethylbenzidine(TMB).Enzymeactivityismeasuredspectrophotometricallybyrecordingabsorbancereadingsat450nm.Increasesinabsorbencyaredirectlyproportionaltotheamountof
      GFAPproteincapturedfromthesample.ThereforetheproteinconcentrationofunknownscanbederivedbyinterpolationusingastandardcurvegeneratedinthesameassaywithreferencestandardsofknownconcentrationsofGFAP.
      AlternateNames
      • GFAP
      • GlialFibrillaryAcidicProteinELISA
      MaterialsRequiredbutNotDelivered1.Absorbentpaperorcloth
      2.Conventional,multi-channel,orrepeatingPipettes
      3.Reagentreservoirs
      4.Orbitalmicrotiterplateshaker/washer(optional)
      5.GraduatedSEROlogicalpipettes
      6.Microtiterplatereadercapableofreadingabsorbencyat450nm
      7.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
      8.Microfugetubesforstandardandsampledilutions
      9.Mechanicalvortex
      10.Milli-Q™Water
      ProductInformation
      Components
      • 96-WellAnti-GFAPCoatedPlate(CS206352):1Plate
      • 1XAssayBuffer(CS206357):40mL
      • 10XWashBuffer(CS206360):50mL
      • GFAPProteinStandard(CS206361):1Vial
      • GFAPQualityControl#1(CS206351):1Vial
      • GFAPQualityControl#2(CS206475):1Vial
      • BiotinylatedAnti-GFAPDetectionAntibody(CS206356):12mL
      • EnzymeSolution(CS206358):12mL
      • TMBSolution(CS202682):10mL
      • StopSolution(60193):12mL
      • PlateSealers(CS200443):10Sheets
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStorage:TheGFAPELISAkitisshippedoniceandshouldbestoredat2-8°Cuponarrival.
      StABIlity:Kitcomponentsarestableforaminimumof6monthsfromthedateofreceiptifstoredand
      handledasdescribedabove.
      Applications
      KeyApplications
      • ELISA
      BiologicalInformation
      SpeciesReactivity
      • Mouse
      • Rat
      • Human
      AnalytesAvailable
      • GFAP
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesoneofthemajorintermediatefilamentproteinsofmatureastrocytes.Itisusedasamarkertodistinguishastrocytesfromotherglialcellsduringdevelopment.MutationsinthisgenecauseAlexanderdisease,araredisorderofastrocytesinthecentralnervoussystem.Alternativesplicingresultsinmultipletranscriptvariantsencodingdistinctisoforms.
      GeneSymbol
      • GFAP
      UniProtNumber
      UniProtSummaryFUNCTION:GFAP,aclass-IIIintermediatefilament,isacell-specificmarkerthat,duringthedevelopmentofthecentralnervoussystem,distinguishesastrocytesfromotherglialcells.
      SUBCELLULARLOCATION:Cytoplasm.Note:Associatedwithintermediatefilaments.
      TISSUESPECIFICITY:Expressedincellslackingfibronectin.
      PTM:PhosphorylatedbyPKN1.
      SIMILARITY:Belongstotheintermediatefilamentfamily.
      PhysicochemicalInformation
      Sensitivity
      • 1.5ng/mL
      StandardCurveRange
      • 1.5to100ng/mL
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    蚂蚁淘生物188，秉承蚂蚁淘一贯的严谨态度，由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后，保障正品优质。以“快速好省，为科研提供好产品、好价格”为理念，直接链接原厂家，从全国各地原制造商严格挑选188款科研精品，剔除品牌溢价，188生物新电商，把好的产品带给科研！  力求给你最优质的商品。
  • Q：生物188产品正品保障吗？
    A：生物188质量把控人员具有十年的从业经验，在业界享有良好的口碑；自营商城，直接从厂家采购， 自己的团队负责国际物流和清关，中间没有第三方，所有流程严格把控，100%保证正品，假一罚十。

    Q：下单后可以修改订单吗？
    A：下单后的商品付款之前可以修改；订单付款成功，需要联系我们客服进行修改；客服电话：4000-520-616

    Q：可以开发票吗？
    A：本网站所售商品都是正规清关，均开具16%正规发票，发票金额含配送费金额，另有说明的除外。

    Q：商品几天可以发货？
    A：生物188商品，全部现货销售，付款后即可发货,一般一周内送达！

    Q：如何联系商家？
    A：有任何问题够可以电话咨询我们，全国24小时免费服务热线：4000-520-616 或联系我们的在线客服QQ：1570468124

    Q：收到的商品少了/发错了怎么办？
    A：同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是 部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

    Q：退换货/维修需要多长时间？
    A：一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、 数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

客户服务

在线客服
用户反馈

何为188

极简而严谨，我们仅销售188款生物医学科研用品，款款都是爆款；因为少所以聚焦，聚焦甄选每一款产品，聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知

  • 品质甄选，正品保证

  • 自营电商，厂家直采

  • 极简主义，188精品

常见问题 | 购买相关 | 配送与验收 | 支付问题 | 售后与服务 | 服务协议 | 隐私政策 | 何为188 | 中国智造 | 联系我们
友情链接： Adventbio中国 | Polysciences第二代聚合物转染 | Iduron公司 | 华东地区代理echelon | AshtaChemicals产品代理 | Dextra Laboratories | lucerna technologies | 蚂蚁淘 | 广州飞迪生物科技有限公司

公司版权所有 © 2005-2021 苏州蚂蚁淘生物科技有限公司 苏ICP备17049038号-10