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NEB/pCLIPf Vector/20 µg/N9215S

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货号: N9215S

品牌: NEB

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- pCLIP_f Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of CLIP-tag protein fusions in mammalian cells. This plasmid encodes CLIP_f, a CLIP-tag protein, which is expressed under control of the CMV promoter. The expression vector has an IRES (internal ribosome entry site) and a neomycin resistance gene downstream of the CLIP_f for the efficient selection of stable transfectants. pCLIP_f Vector contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the CLIP_f.The CLIP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The CLIP-tag is a small protein based on human O⁶-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link.pCLIP_f contains an improved version of CLIP-tag, termed CLIP_f. CLIP_f displays faster kinetics in in vitro labeling and fast, specific and efficient labeling in live and fixed cell applications, thereby rendering it a desired research tool for analysis of protein dynamics.Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling.
  There are two steps to using this system: sub cloning and expression of the protein of interest as a CLIP_f fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Cloning and expression of CLIP_f fusion proteins are described in this document. The labeling of the fusion proteins with CLIP-tag substrates is described in the instructions supplied with the CLIP-tag substrates.
  Cloning Region of pCLIPf
  Unique restriction sites in the regions flanking the CLIP_f gene are displayed above the coding strand. The complete sequence for pCLIP_f and the control plasmids can be downloaded here .
  This product is related to the following categories:
  Cellular Analysis Vectors Products,
  DNA Plasmids & Substrates Products,
  Protein Labeling Products
  This product can be used in the following applications:
  CLIP Cell,
  CLIP Surface,
  In vivo Imaging,
  Pulse Chase,
  Receptor Internalization,
  Protein Localization,
  Protein Labeling Snap Clip
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