pTXB1 is an E. coli expression vector in the IMPACT™ Kit (NEB #E6901) (1,2). It is designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain (27 kDa) (2,3,4,5). The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein. pTXB vectors are recommended for use in intein-mediated protein ligation and C-terminal labeling (2). This double stranded vector is 6,706 base pairs in length.
DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.
This product is related to the following categories:
IMPACT System,
Bacterial E. coli Protein Expression,
Chitin Purification (CBD-tag),
DNA Plasmids & Substrates Products,
Protein Purification,
Protein Expression
This product can be used in the following applications: