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Millipore/MABN50 | Anti-Olig2 Antibody, clone 211F1.1/MABN50/100 µg
  • Millipore/MABN50 | Anti-Olig2 Antibody, clone 211F1.1/MABN50/100 µg

Millipore/MABN50 | Anti-Olig2 Antibody, clone 211F1.1/MABN50/100 µg

价格: ¥4440.00 市场价: 7400.00

货号: MABN50
品牌: Millipore
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    • Description
      CatalogueNumberMABN50
      DescriptionAnti-Olig2Antibody,clone211F1.1
      AlternateNames
      • oligodendrocytelineagetranscriptionfactor2
      • oligodendrocyte-specificbHLHtranscriptionfactor2
      • humanproteinkinaseC-bindingproteinRACK17
      • ProteinkinaseC-bindingprotein2
      • proteinkinaseCbindingprotein2
      • ClassBbasichelix-loop-helixprotein1
      • ProteinkinaseC-bindingproteinRACK17
      • basicdomain,helix-loop-helixprotein,classB,1
      BackgroundInformationOligodendrocytelineagetranscriptionfactor2(Olig2)isaproteinbelongingtogroupAoftheOLIGfamilyofbasichelix-loop-helixtranscriptionfactors(bHLH).bHLHproteinsaredividedinto6groups(A-F),andcharacterizedbytheirbiochemicalpropertiesandstructure.Olig2isclassifiedasgroupAduetoitsABIlitytoformheterodimerswithotherbHLHproteinsandbindingtotheEbox.Olig2hasacrucialroleduringdevelopmentinspecifyingthefinallocationofmotorneuronsandoligodendrocytesinthespinalcord,alongwiththedevelopmentwithinthehindbrainofsomaticmotorneurons.Olig2expressionisobservedwithinoligodendrocytesandindevelopingastrocytes.RecentstudieshaveshownstrongexpressionofOlig2inhumanoligodendrogliomas,primarytumorswithinthecentralnervoussystem,andmoderateexpressioninastrocytomas.TheseobservationshaveledtotheproposalofOlig2havingpotentialasanoligodendrogliomadiagnosticMarker.
      ProductInformation
      FormatPurified
      Control
      • Mousebrainlysate
      PresentationPurifiedmousemonoclonalIgG2aκinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      Applications
      ApplicationDetectOlig2usingthisAnti-Olig2Antibody,clone211F1.1validatedforuseinWB,IC,IH&IP.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      ApplicationNotesImmunohistochemistryAnalysis:ThisantibodyhasbeenshowntodetectOlig2incoronalbraintissueasreportedbyanindependentlaboratory(Cai,J.,etal.(2007).Development.134:1887-1899).

      ImmunocytochemistryAnalysis:ThisantibodyhasbeenshowntodetectOlig2,asreportedbyanindependentlaboratory(Lee,S.K.,etal.(1995).Genes&Dev.19:282-294).

      ImmunoprecipitationAnalysis:ThisantibodyhasbeenshowntodetectOlig2,asreportedbyanindependentlaboratory(Lee,S.K.,etal.(1995).Genes&Dev.19:282-294).
      BIOLOGicalInformation
      ImmunogenRecombinantproteincorrespondingtohumanOlig2.
      EpitopeUnknown
      Clone211F1.1
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityThisantibodyrecognizesOlig2.
      IsotypeIgG2aκ
      SpeciesReactivity
      • Mouse
      • Human
      • Rat
      SpeciesReactivityNoteDemonstratedtoreactwithmouse.Predictedtoreactwithhumanandratbasedon100%sequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • OLIG2
      • OLIGO2
      • RACK17
      • PRKCBP2
      • Oligo2
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:Requiredforoligodendrocyteandmotorneuronspecificationinthespinalcord,aswellasforthedevelopmentofsomaticmotorneuronsinthehindbrain.CooperateswithOLIG1toestablishthepMNdomainoftheembryonicneuraltube.AntagoNISTofV2interneuronandofNKX2-2-inducedV3interneurondevelopment(Bysimilarity).

      SIZE:323aminoacids;32385Da

      SUBUNIT:InteractswithNKX2-2(Bysimilarity).

      SUBCELLULARLOCATION:Nucleus(Bysimilarity).Cytoplasm(Bysimilarity).Note=TheNLScontainedinthebHLHdomaincouldbemaskedinthenativeformandtranslocationtothenucleuscouldbemediatedbyinteractioneitherwithclassEbHLHpartnerproteinorwithNKX2-2(Bysimilarity).

      TISSUESPECIFICITY:Expressedinthebrain,inoligodendrocytes.Stronglyexpressedinoligodendrogliomas,whileexpressionisweaktomoderateinastrocytomas.Expressioninglioblastomashighlyvariable.

      DOMAIN:ThebHLHisessentialforinteractionwithNKX2-2(Bysimilarity).DISEASE:SwissProt:Q13516#AchromosomalaberrationinvolvingOLIG2maybeacauseofaformofT-cellacutelymphoblasticleukemia(T-ALL).Translocationt(14;21)(q11.2;q22)withTCRA.

      SIMILARITY:Contains1basichelix-loop-helix(bHLH)domain.
      MolecularWeight~37kDaobserved
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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