Mouse monoclonal antibody raised against synthetic peptide of ACTB.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of ACTB.
Host:
Mouse
Reactivity:
Chicken, Human, Mouse, Rabbit, Rat
Specificity:
Recognizes native and denatured forms of beta-Actin (~42 KDa).
Form:
Liquid
Purification:
Protein A purification
Concentration:
1 mg/mL
Isotype:
IgG2b
Recommend Usage:
Western Blot (1:1000-1:10000)Immunohistochemistry (1:500-1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Applications
Western Blot (Tissue lysate)
Western blot analysis of 20 ug/lane tissue lysate from human (1), mouse (2), rat (3) and rabbit (4). Blotting with ACTB monoclonal antibody, clone BA3R (Cat # MAB9743) at 1:1000 dilution.