Mouse monoclonal antibody raised against partial recombinant Ahi1.
Amount:
100 uL
Immunogen:
Recombinant protein corresponding to C-terminus of mouse Ahi-1.
Host:
Mouse
Reactivity:
Mouse
Specificity:
This antibody only specifically reacts with murine Ahi-1.
Form:
Liquid
Purification:
Affinity purification
Concentration:
1 ug/uL
Isotype:
IgG1
Recommend Usage:
Western Blot (1:1000-1:2000)Immunoprecipitation (1-2 ug/200-800 ug protein)IHC (1:200-1:1000)The optimal working dilution should be determined.
Storage Buffer:
In PBS, pH 7.4 (0.02% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Applications
Western Blot (Recombinant protein)
Immunoprecipitation and Western blot analysis in Lane 1: parental BaF3 cells, Lane 2: BaF3+BCR-ABL (BaF3 cells transduced with BCR-ABL), Lane 3: BaF3+BCR-ABL+mAhi-I (M1, BaF3 cells transduced with both BCR-ABL and mouse Ahi-1, M1 is a clonal cell line), Lane 4: BaF3+BCR-ABL+mAhi-1 (C2, BaF3 cells transduced with both BCR-ABL and mouse Ahi-1, C2 is a clonal cell line) with Ahi1 monoclonal antibody, clone 645s3 (Cat # MAB8962).
Immunohistochemistry
Immunofluorescence
Immunofluorescence analysis in Ba/F3 cells with Ahi1 monoclonal antibody, clone 645s3( Cat # MAB8962 ) at 1 : 200 dilution.
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