Western Blot (1:1000-1:4000)Immunofluorescence (1:100-1:400)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Nebulin regulates the assembly and lengths of the thin filaments in striated muscle.McElhinny AS, Schwach C, Valichnac M, Mount-Patrick S, Gregorio CC.J Cell Biol. 2005 Sep 12;170(6):947-57.
2.
bPAK-interacting exchange factor may regulate actin cytoskeleton through interaction with actin.Lee CS, Kim KY, Im JB, Choi JW, Kim HK, Park JS, Shin EY, Kim SR, Kim EG.Exp Mol Med. 2004 Dec 31;36(6):582-7.
Applications
Western Blot (Cell lysate)
Western blot analysis of ACTB in 293 cells. 20 ug of total lysate was bloted with ACTB monoclonal antibody, clone G043 (Cat # MAB8182).
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