Mouse monoclonal antibody raised against 5-Methylcytosine.
Immunogen:
5-Methylcytosine conjugated with BSA.
Host:
Mouse
Form:
Liquid
Isotype:
IgM
Recommend Usage:
Immunocytochemistry (1:50-1:100)Western Blot (~1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol).
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Publication Reference
1.
The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA.Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo TA, Shinga J, Mizutani-Koseki Y, Toyoda T, Okamura K, Tajima S, Mitsuya K, Okano M, Koseki H.Nature. 2007 Dec 6;450(7171):908-12. Epub 2007 Nov 11.
2.
A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes.Nishiyama R, Ito M, Yamaguchi Y, Koizumi N, Sano H.Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5925-30.
3.
Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine.Sano H, Imokawa M, Sager R.Biochim Biophys Acta. 1988 Nov 10;951(1):157-65.
Applications
Immunocytochemistry
Immunofluorescence
Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamydomonas me-1 cells.Left : DAPI-stained cells.Middle : Cells stained with 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766) and FITC-conjugated 2nd antibody.Right : Merged image Chloroplast DNA is exclusively methylated in gamete cells.
Immunofluorescence
Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766). Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs.
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