Detects ~50-60KDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
Western Blot (0.1 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase.Hughes K, Edin S, Antonsson A, Grundström T.J Biol Chem. 2001 Sep 21;276(38):36008-13. Epub 2001 Jul 24.
2.
Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation.Makhinson M, Chotiner JK, Watson JB, O"Dell TJ.J Neurosci. 1999 Apr 1;19(7):2500-10.
3.
Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation.Barria A, Muller D, Derkach V, Griffith LC, Soderling TR.Science. 1997 Jun 27;276(5321):2042-5.
Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunofluorescence staining in cultured rat cortical neurons with Camk2a monoclonal antibody, clone 6G9 (Cat # MAB6626).
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
Application Image
Western Blot
Immunohistochemistry
Immunofluorescence
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Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
Gene Information
Entrez GeneID:
25400
Gene Name:
Camk2a
Gene Alias:
PK2CDD,PKCCD
Gene Description:
calcium/calmodulin-dependent protein kinase II alpha
Gene Ontology:
Hyperlink
Other Designations:
Ca2+/calmodulin-dependent protein kinase II alpha,alpha CaM kinase II,calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha,calcium/calmodulin-dependent protein kinase II alpha subunit
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