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Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL
  • Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

价格: ¥4308.00 市场价: 7180.00

货号: MAB5304
品牌: Millipore
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    • Description
      CatalogueNumberMAB5304
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-GlutamateAntibody
      ProductInformation
      FormatPurified
      PresentationLiquidinPBScontaining10mMsodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6monthsafterdate.
      Applications
      ApplicationAnti-GlutamateAntibodydetectslevelofGlutamate&hasbeenpublished&validatedforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:2,500-1:10,000usingfreefloatingsectionsbythePAPtechniqueonrathippocampus.

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      PROTOCOLforGlutamateDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain.

      1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded.

      SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2.

      SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5.

      2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min.

      3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC).

      4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused.

      5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride.

      6.APPLICATIONOFGLUTAMATEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+2-8°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure.

      7.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-mouseinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor1-2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionC.

      8.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionCfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionC.

      9.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia.

      Forresearchuseonly;notforuseasadiagnostic.
      BIOLOGicalInformation
      ImmunogenGlutamate-Glutaraldehyde-BSA.
      HostMouse
      SpecificityGlutamate

      Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds:

      CompoundCross-reactivity

      Glutamate-G-BSA1

      Aspartate-G-BSA1/100,000

      GABA-G-BSA1/100,000

      Glutamate1/100,000

      Abbreviations:

      (G)Glutaraldehyde

      (BSA)BovineSerumAlbumin

      NOTE:AntibodyreactivityREQUIRESglutaraldehydefixationthussomeglutaraldehyde(0.5%-2.0%)needstobeincludedinthetissuefixationprocedureinorderfortheproperreactivity.
      IsotypeIgM
      SpeciesReactivity
      • Rat
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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