Immunogen | Purifiedtyrosinehydroxylase(EC1.14.16.2)fromaratpheochromocytoma(2). |
Clone | clone2/40/15 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Mouse |
Specificity | Tyrosinehydroxylase(TH)catalyzestherate-determininginitialstepinthebiosynthesisofcatecholaminessuchasdopamine,noradrenalineandadrenaline.Thereforetheantibodyisusedtostaindopaminergeousandadrenergeousneuronsaswellasthechromaffinetissueoftheadrenalmedulla.CelllinessuchasthePC12line,whichisderivedfromaratpheochromocytomaalso,showpositivereactions.Inimmunoblotsofadrenalmedullatissue,theantibodyrecognizesasingleproteinbandinthemolecularweightrangeof56-60kD. |
Isotype | IgG2a |
SpeciesReactivity | |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Tyrosinehydroxylaseisinvolvedintheconversionoftyrosinetodopamine.Astherate-limitingenzymeinthesynthesisofcatecholamines,tyrosinehydroxylasehasakeyroleinthephysiologyofadrenergicneurons. |
GeneSymbol | |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P07101#Playsanimportantroleinthephysiologyofadrenergicneurons. COFACTOR:Fe(2+)ion. SIZE:528aminoacids;58524Da TISSUESPECIFICITY:Mainlyexpressedinthebrainandadrenalglands. DISEASE:SwissProt:P07101#DefectsinTHarethecauseofautosomalrecessiveSegawasyndrome[MIM:605407];alsoknownasDOPA-responsivedystonia.Typically,itbeginsinchildhoodoradolescencewithprogressivedifficultyinwalkingand,insomecases,spasticity.Somecasespresentwithparkinsoniansymptomsininfancyandarereferredtoasautosomalrecessiveinfantileparkinsonism. SIMILARITY:SwissProt:P07101##Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily. |