Mouse monoclonal antibody raised against 5-methyl Cytosine.
Immunogen:
Ovalbumine conjugated with 5-methyl Cytosine.
Host:
Mouse
Reactivity:
Human, Mouse
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (0.1-0.3 ug/mL)Dot Blot (1-2 ug/mL)Immunofluorescence (1:100)Flow Cytometry (1-2 ug/mL)Methylated DNA Immunoprecipitation (1-5 ug/IP)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS
Storage Instruction:
Aliquot and store at -80°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Applications
Immunofluorescence
Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952).A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um.B. Enlarged picture corresponding to a region from the left panel (as indicated in A).C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B).
Immunoprecipitation
MeDIP assay was performed using fragmented genomic DNA from the MCF7 breast cancer cells, 5-mC monoclonal antibody, clone b (Cat # MAB4952) and optimized PCR primer sets for qPCR of the indicated regions.1 ug of antibody was used per IP experiment.The hypermethylated p14 INK4A intronic CpG island and Alu repeats are clearly recovered, whereas the non-methylated promoter-CpG island of the p14 INK4A gene is not (red bars).One IP negative control (no antibody added) was included in the assay, the background is extremely low (white bars).
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