Mouse monoclonal antibody raised against Adeno-associated Virus (AAV), VP1, VP2 and VP3.
Immunogen:
Adeno-associated virus capsid proteins and virus particles.
Host:
Mouse
Reactivity:
Viruses
Specificity:
Reacts with free VP1, VP2 and VP3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope identified a.a. 726-733 (C-terminus; common to all 3 VP proteins) as the specific binding region. This antibody is also useful for characterization of different stages of infection. AAV-2 found in human and monkey.
Form:
Lyophilized
Purification:
Protein A Chromatography
Isotype:
IgG1
Recommend Usage:
Affinity ChromatographyImmunofluorescenceImmunohistochemistry (1:10)Immunoprecipitation (Mainly Free VP Proteins)Western BlotThe optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store at 4°C on dry atmosphere for short term. For long term storage, aliquot and store at -20°C. After reconstitution with 1 mL of phosphate buffered saline, store at - 20°C.Aliquot to avoid repeated freezing and thawing. Centrifuge before opening to ensure complete recovery of vial contents.
Datasheet:
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Publication Reference
1.
Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection.Wobus CE, Hugle-Dorr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA.J Virol. 2000 Oct;74(19):9281-93.
2.
Subcellular compartmentalization of adeno-associated virus type 2 assembly.Wistuba A, Kern A, Weger S, Grimm D, Kleinschmidt JA.J Virol. 1997 Feb;71(2):1341-52.
3.
Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins.Wistuba A, Weger S, Kern A, Kleinschmidt JA.J Virol. 1995 Sep;69(9):5311-9.
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