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Millipore/MAB388-100UG | Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1/MAB388-100UG/100 µg
  • Millipore/MAB388-100UG | Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1/MAB388-100UG/100 µg

Millipore/MAB388-100UG | Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1/MAB388-100UG/100 µg

价格: ¥4440.00 市场价: 7400.00

货号: MAB388-100UG
品牌: Millipore
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    • Description
      CatalogueNumberMAB388-100UG
      ReplacesMAB388
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-MyelinProteolipidProteinAntibody,CT,clonePLPC1
      AlternateNames
      • PLP
      ProductInformation
      FormatPurified
      Control
      • Brain
      PresentationPurifiedmouseimmunoglobulininPBS,pH7.2containing0.09%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationDetectMyelinProteolipidProteinusingthisAnti-MyelinProteolipidProteinAntibody,C-terminus,clonePLPC1validatedforuseinIH,IH(P)&WB.
      KeyApplications
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesWesternBlotting:detectsa23-25kDaproteinandDM20a20kDaprotein;PLPcanaggregatecausingHMWbandstosometimesappearincertainpreparations.

      Immunocytochemistry:4%PFAfixedcells,5-10minutesroomtemperature.Use0.1%tritoninblockonlytoallowantibodyaccesstoC-terminalepitope.

      Immunohistologyonfreshfrozensectionsor4%PFAfixed,frozentissues.Epitopeisinternal,use0.1-0.2%tritonX-100intheblockingstepforpermeABIlization.

      Immunohistochemistryinparaffin:worksbestwithlightlyfixedtissues,orBouin"sfixedmaterial,althoughtherearereportsoftrADItionalformalinfixationalsoworkingwithantigenrecovery.{Mathis,etal2003}.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenMyelinproteolipidprotein,syntheticpeptideCGRGTKFcorrespondingtotheC-terminalpeptide.
      EpitopeC-terminus
      ClonePLPC1
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityReactswithmyelinproteolipidproteininmanymammalianspecies.Showscross-reactivitytoDM20.
      IsotypeIgG2a
      SpeciesReactivity
      • Mammals
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesatransmembraneproteolipidproteinthatisthepredominantmyelinproteinpresentinthecentralnervoussystem.Itmayplayaroleinthecompaction,stabilization,andmaintenanceofmyelinsheaths,aswellasinoligodendrocytedevelopmentandaxonalsurvival.MutationsinthisgenecauseX-linkedPelizaeus-Merzbacherdiseaseandspasticparaplegiatype2.Alternativelysplicedtranscriptvariantsencodingdistinctisoformsorhavingdifferent5"UTRs,havebeenidentifiedforthisgene.
      GeneSymbol
      • PLP
      • PMD
      • MMP
      • SPG2
      • PLP/DM20
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P60201#Thisisthemajormyelinproteinfromthecentralnervoussystem.Itplaysanimportantroleintheformationormaintenanceofthemultilamellarstructureofmyelin.
      SIZE:277aminoacids;30,077Da
      SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein.
      PTM:Lipoprotein,Palmitate
      DISEASE:DefectsinPLP1arethecauseofPelizaeus-Merzbacherdisease(PMD)[MIM:312080].PMDisanX-linkedneurologicdisorderofmyelinmetabolism.Itischaracterizedbyearlyimpairmentofmotordevelopment(duringthefirstthreemonthsoflife)and,later,bythedevelopmentofabnormalmovementsandprogressivespasticparaplegia.
      SIMILARITY:Belongstothemyelinproteolipidproteinfamily.
      MolecularWeight23-25kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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