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Millipore/MAB3420 | Anti-Tau-1 Antibody, clone PC1C6/MAB3420/100 µg
  • Millipore/MAB3420 | Anti-Tau-1 Antibody, clone PC1C6/MAB3420/100 µg

Millipore/MAB3420 | Anti-Tau-1 Antibody, clone PC1C6/MAB3420/100 µg

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货号: MAB3420
品牌: Millipore
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    • Description
      CatalogueNumberMAB3420
      ReplacesAB1512
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Tau-1Antibody,clonePC1C6
      BackgroundInformationTau,amicrotubulebindingproteinwhichservestostABIlizemicrotubulesingrowingaxons,isfoundtobehyperphosphorylatedinpairedhelicalfilaments(PHF),themajorfibrouscomponentofneurofibrillarylesionsassociatedwithAlzheimer’sdisease.HyperphosphorylationofTauisthoughttobethecriticaleventleADIngtotheassemblyofPHF.SixTauproteinisoformshavebeenidentified,allofwhicharephosphorylatedbyglycogensynthasekinase3(GSK3).Cellularandsubcellularlocalization:Insitu,anti-tau-1hasastringentspecificityfortheaxonsofneurons.Theantibodydoesnotstainthecellbodiesordendritesofneurons,nordoesitstainanyothercelltype(4).However,thisinvivointracellularspecificityisnotmaintainedinculture:anti-tau-1stainstheaxon,cellbodies,anddendritesofrathippocampalneuronsgrowninculture(5).Thespecificityofanti-tau-1wasoriginallythoughttorepresenttherestrictedexpressionoftautoaxons.Laterstudiesrevealedthatthisspecificityisdependantonthestateofphosphorylation.Indephosphorylatedsamples(samplestreatedwithalkalinephosphatase)anti-tau-1stainsastrocytes,perineuronalglialcells,andtheaxons,cellbodiesanddendritesofneurons,whileinuntreatedsamples,anti-tau-1stainsonlyaxons(6).(Theepitoperecognizedbyanti-tau-1isprobablyatornearaphosphorylatedsite.)
      ProductInformation
      FormatPurified
      Control
      • Alzheimer"sbraintissue(dephosphorylationwithalkalinephosphataseisrecommendedforstainingneurofibrillarytanglesinAlzheimer’sbraintissue)orhumanT98Gglioblastomacells
      Presentation0.02Mphosphatebuffer,pH7.6,0.25MNaCl,and0.1%sodiumazide
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-Tau-1Antibody,clonePC1C6isanantibodyagainstTau-1foruseinIH&WBwithmorethan65productcitations.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesWesternblot:Bovinebrainmicrotubuleproteinspurifiedbytwocyclesofassemblyanddisassembly(9)aredissolvedinSDS-PAGEsamplebuffer.Fivemicrogramsofthemicrotublepreparationperlaneisloadedontoa4%to20%SDS-PAGEgradientgelalongsidemolecularweightMarkers(14.3-200kD).Afterseparationbyelectrophoresis,theproteinsareblottedontonitrocellulose.Tauisdetectedasaseriesof5bands(52-68kD)withapproximately5ng/mLofanti-tau-1.

      Immunohistochemistry:5μg/mL;stainsaxonsintissueprimarily,howeverincultureTauexpressionisnotrestrictedtojustaxons.

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      ImmunohistochemistryProtocol

      Dephosphorylationoftissuesections(optional)

      DephosphorylationwithalkalinephosphataseisrecommendedforstainingneurofibrillarytanglesinAlzheimer"sbraintissuewithanti-tau-1(6).Thistreatmentchangesthestainingpatternofanti-tau-1toincludecellbodies,dendritesandaxonsofneurons.Inuntreatedsamples,anti-tau-1stainsaxonsonly.

      1.Incubatetissuesectionsat+32°Cfor2.5hourswithconstantagitationinthefollowingsolution:100mMTris-HCl,pH8.0;130units/mLalkalinephosphatase,1mMPMSF,10μg/mLpepstatinand10μg/mLleupeptin.

      2.Rinsesectionstwice,3minperrinse,with100mMTris-HCl,pH8.0.

      Anti-tau-1staining

      1.Blocknon-specificbindingbyincubatingsectionsinPBScontaining1%(v/v)normalanimalserum,and0.03%(w/v)TritonX-100.Theanimalserumshouldbefromthesamespeciesasthesecondaryantibody.

      2.Rinse3timeswithPBS,3minperrinse.

      3.Incubatesectionswithanti-tau-1,approximately5μg/mL,dilutedinPBScontaining1%(v/v)normalanimalserum.

      4.WashwithPBS,changingthesolution3timesovera3minperiod.

      5.Detectwithastandardsecondaryantibodydetectionsystem(10-13).
      BIOLOGicalInformation
      ImmunogenPurifieddenaturedbovinemicrotubuleassociatedproteins.
      ClonePC1C6
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityBindstoallknownelectrophoreticspeciesoftauinhuman,ratandbovinebrain(one-dimensionalSDS-PAGE).HoweverthereissomeunphosphorylatedbiaswithclonePC1C6asitseemtorecognizeonlydephosphorylatedserinesitesat195,198,199,and202{Szendrei,etal1993;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi-cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7680727}.AlsoseeBillingsley&Kincaid,1997BiochemJ323:577-591foradditionalmappinginformationonPC1C6.
      IsotypeIgG2a
      SpeciesReactivity
      • Bovine
      • Human
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesthemicrotubule-associatedproteintau(MAPT)whosetranscriptundergoescomplex,regulatedalternativesplicing,givingrisetoseveralmRNAspecies.MAPTtranscriptsaredifferentiallyexpressedinthenervoussystem,dependingonstageofneuronalmaturationandneurontype.MAPTgenemutationshavebeenassociatedwithseveralneurodegenerativedisorderssuchasAlzheimer"sdisease,Pick"sdisease,frontotemporaldementia,cortico-basaldegenerationandprogressivesupranuclearpalsy.
      GeneSymbol
      • MAPT
      • MTBT2
      • MAPTL
      • tau
      • FTDP-17
      • MSTD
      • TAU
      • FLJ31424
      • MTBT1
      • PHF-tau
      • DDPAC
      • MGC138549
      • PPND
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P10636#Promotesmicrotubuleassemblyandstability,andmightbeinvolvedintheestablishmentandmaintenanceofneuronalpolarity.TheC-terminusbindsaxonalmicrotubuleswhiletheN-terminusbindsneuralplasmamembranecomponents,suggestingthattaufunctionsasalinkerproteinbetweenboth.Axonalpolarityispredeterminedbytaulocalization(intheneuronalcell)inthedomainofthecellbodydefinedbythecentrosome.TheshortisoformsallowplasticityoftheCytoskeletonwhereasthelongerisoformsmaypreferentiallyplayaroleinitsstabilization.
      SIZE:758aminoacids;78878Da
      SUBUNIT:InteractswithPSMC2throughSQSTM1(Bysimilarity).InteractswithSQSTM1whenpolyubiquitinated.
      SUBCELLULARLOCATION:Cytoplasm,cytosol.Cellmembrane.Note=Mostlyfoundintheaxonsofneurons,inthecytosolandinassociationwithplasmamembranecomponents.
      TISSUESPECIFICITY:Expressedinneurons.IsoformPNS-tauisexpressedintheperipheralnervoussystemwhiletheothersareexpressedinthecentralnervoussystem.DEVELOPMENTALSTAGE:Four-repeat(typeII)tauisexpressedinanadult-specificmannerandisnotfoundinfetalbrain,whereasthree-repeat(typeI)tauisfoundinbothadultandfetalbrain.
      DOMAIN:SwissProt:P10636Thetau/MAPrepeatbindstotubulin.TypeIisoformscontain3repeatswhiletypeIIisoformscontain4repeats.
      PTM:PhosphorylationatserineandthreonineresiduesinS-PorT-Pmotifsbyproline-directedproteinkinases(PDPK:CDC2,CDK5,GSK-3,MAPK)(only2-3sitesperproteinininterphase,seven-foldincreaseinmitosis,andinPHF-tau),andatserineresiduesinK-X-G-SmotifsbyMAP/microtubuleaffinity-regulatingkinase(MARK)inAlzheimerdiseasedbrains.Phosphorylationdecreaseswithage.Phosphorylationwithintau"srepeatdomainorinflankingregionsseemstoreducetau"sinteractionwith,respectively,microtubulesorplasmamembranecomponents.PhosphorylationonSer-610,Ser-622,Ser-641andSer-673inseveralisoformsduringmitosis.&Polyubiquitinated.RequiresfunctionalTRAF6andmayprovokeSQSTM1-dependentdegradationbytheproteasome(Bysimilarity).PHF-taucanbemodifiedbythreedifferentformsofpolyubiquitination."Lys-48"-linkedpolyubiquitinationisthemajorform,"Lys-6"-linkedand"Lys-11"-linkedpolyubiquitinationalsooccur.&GlycationofPHF-tau,butnotnormalbraintau.Glycationisanon-enzymaticpost-translationalmodificationthatinvolvesacovalentlinkagebetweenasugarandanaminogroupofaproteinmoleculeformingketoamine.Subsequentoxidation,fragmentationand/orcross-linkingofketoamineleadstotheproductionofadvancedglycationendproducts(AGES).GlycationmayplayaroleinstabilizingPHFaggregationleadingtotangleformationinAD.
      DISEASE:SwissProt:P10636#InAlzheimerdisease,theneuronalcytoskeletoninthebrainisprogressivelydisruptedandreplacedbytanglesofpairedhelicalfilaments(PHF)andstraightfilaments,mainlycomposedofhyperphosphorylatedformsofTAU(PHF-TAUorADP-TAU).&DefectsinMAPTareacauseoffrontotemporaldementiaandparkinsonismlinkedtochromosome17(FTDP17)[MIM:600274,172700];alsocalledfrontotemporaldementia(FTD)orhistoricallytermedPickcomplex.Thisformoffrontotemporaldementiaischaracterizedbypreseniledementiawithbehavioralchanges,deteriorationofcognitivecapacitiesandlossofmemory.Insomecases,parkinsoniansymptomsareprominent.Neuropathologicalchangesincludefrontotemporalatrophyoftenassociatedwithatrophyofthebasalganglia,substantianigra,amygdala.Inmostcases,proteintaudepositsarefoundinglialcellsand/orneurons.&DefectsinMAPTareacauseofpallido-ponto-nigraldegeneration(PPND)[MIM:168610].Theclinicalfeaturesincludeocularmotilityabnormalities,dystoniaandurinaryincontinence,besidesprogressiveparkinsonismanddementia.&DefectsinMAPTareacauseofcorticobasaldegeneration(CBD).Itismarkedbyextrapyramidalsignsandapraxiaandcanbeassociatedwithmemoryloss.NeuropathologicfeaturesmayoverlapAlzheimerdisease,progressivesupranuclearpalsy,andParkinsondisease.&DefectsinMAPTareacauseofprogressivesupranuclearpalsy(PSP)[MIM:601104,260540];alsoknownasSteele-Richardson-Olszewskisyndrome.PSPischaracterizedbyakinetic-rigidsyndrome,supranucleargazepalsy,pyramidaltractdysfunction,pseudobulbarsignsandcognitivecapacitiesdeterioration.Neurofibrillarytanglesandgliosisbutnoamyloidplaquesarefoundindiseasedbrains.Mostcasesappeartobesporadic,withasignificantassociationwithacommonhaplotypeincludingtheMAPTgeneandtheflankingregions.Familialcasesshowanautosomaldominantpatternoftransmissionwithincompletepenetrance;geneticanalysisofafewcasesshowedtheoccurrenceoftaumutations,includingadeletionofAsn-613.&DefectsinMAPTmaybeacauseofhereditarydysphasicdisinhibitiondementia(HDDD)[MIM:607485].HDDDisafrontotemporaldementiacharacterizedbyprogressivecognitivedeficitswithmemorylossandpersonalitychanges,severedysphasicdisturbancesleadingtomutism,andhyperphagia.
      SIMILARITY:Contains4Tau/MAPrepeats.
      MolecularWeight5bands(52–68kDa)
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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