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Millipore/MAB326 | Anti-CNPase Antibody, clone 11-5B/MAB326/100 µg
  • Millipore/MAB326 | Anti-CNPase Antibody, clone 11-5B/MAB326/100 µg

Millipore/MAB326 | Anti-CNPase Antibody, clone 11-5B/MAB326/100 µg

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货号: MAB326
品牌: Millipore
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    • Description
      CatalogueNumberMAB326
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CNPaseAntibody,clone11-5B
      AlternateNames
      • 2"3"-CyclicNucleotide3"-Phosphohydrolase
      BackgroundInformationCNPase(2",3"-cyclicnucleotide3"-phosphodiesterase[or-phosphohydrolase],EC3.1.4.37)ispresentinveryhighlevelsinbrainandperipheralnerve.Thisenzymeisfoundalmostexclusivelyinoligo-dendrocytesandSchwanncells,thecellsthatformmyelininthecentralandperipheralnervoussystem,respectively.
      ImmunohistochemicallocalizationofCNPasehasshowntheenzymetoberestrictedtooligodendrocytesandSchwanncells.Theenzymeappearstobedistributedinsingleanddoubleloosewrapsofmyelinandnotincompactmyelinasearlierthoughtbymostinvestigators.CNPaseislocatedontheinnerandouterloopsofmyelin,paranodallyandneartheinnersurfaceoftheoligodendrocytemembrane.Inplaqueregionsofmultiplesclerosispatients,theenzymeisreduced,andwhenCNSmyelinisdecreased,CNPaseisoneoftheearlierproteinstobelostfromthemyelin.Inaddition,anenzymethatisprobablyidenticaltobrainCNPaseislocatedintheouterrodsegmentswithinthevisualsystem,andthisproteinisalsorecognizedbythemonoclonalantibody11-5B.Inmixedhumangliomas,theenzymelevelsarereduced,althoughabout5%oftheoligodendrocytesoccasionallyshownormalpositivestaining.
      ProductInformation
      FormatPurified
      Control
      • WesternBlot:Freshbovinewholebrainextract,mousebrainlysate.
        Immunohistochemistry:Rathippocampustissue,ratspinalcordtissue.
      PresentationPurifiedmousemonoclonalIgG1inbuffercontaining0.02Mphosphatebuffer,0.25MNaClwith0.1%sodiumazide
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8ºCfromdateofreceipt.
      Applications
      ApplicationDetectCNPaseusingthisAnti-CNPaseAntibody,clone11-5BvalidatedforuseinIC,IH,IH(P)&WB.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesImmunocytochemistry:
      Apreviouslotwasusedonprimaryoligodendrocytecultures.

      Immunohistochemistry:
      Apreviouslotwasusedonrathippocampustissueandratspinalcord.
      Immunoblottingofmyelin,theWolfgramproteinfraction,theSN4fraction,tissuesectionsandmixedglialtumors(oligodendrogliomas,etc.)CNPaseI(46kDa)andCNPaseII(48kDa),whicharedifferentiallyregulatedduringdevelopment,withthelargerproteinbeingexpressedearlierthanCNPaseIduringdevelopment.
      Immunohistochemistryonbothfreshfrozenandparaffinembeddedtissue(microwavepretreatment,ctiratepH6.0).

      Immunoblot:
      Immunoblottingofmyelin,theWolfgramproteinfraction,theSN4fraction,tissuesections
      andmixedglialtumors(oligodendrogliomas,etc.)

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenPurifiedhumanbrainCNPase
      Clone11-5B
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityCNPase,48and46kDpolypeptidesbySDS-PAGE.DifferentiatesclearlyoligodendrocytesandSchwanncellsfromneurons,astrocytes,etc.
      IsotypeIgG1
      SpeciesReactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      • Sheep
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • CNP
      • CNPase
      • CNP1
      • EC3.1.4.37
      Non-ReactiveSpecies
      • Chicken
      • GuineaPig
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummarySIZE:421aminoacids;47579Da
      SUBCELLULARLOCATION:Membrane.Melanosome.Note=Firmlyboundtomembranestructuresofbrainwhitematter.IdentifiedbymassspectrometryinmelanosomefractionsfromstageItostageIV.
      SIMILARITY:SwissProt:P09543##Belongstothecyclicnucleotidephosphodiesterasefamily.
      MolecularWeight48&46kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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