当前位置 ： Millipore >>> Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL
Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL
  • Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL

Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL

价格: ¥3072.00 市场价: 5120.00
打印产品信息
货号: MAB305
品牌: Millipore
规格
数量
库存(0)
特别 提示
代购产品：无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
立即购买 加入购物车

收藏商品
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616

  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • Description
      CatalogueNumberMAB305
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CholineAcetyltransferaseAntibody,clone1E6
      AlternateNames
      • ChAT
      • CholineAcetylase
      • CHOACTase
      ProductInformation
      FormatAscites
      Control
      • Braintissue
      PresentationAscitesfluidcontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationDetectCholineAcetyltransferaseusingthisAnti-CholineAcetyltransferaseAntibody,clone1E6validatedforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:100-1:250.Seeimmunohistochmistryprocedurebelow.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      IMMUNOHISTOCHEMISTRYPROCEDURE(PAPTECHNIQUE)FORMAB305,MONOCLONALANTIBODYTOCHOLINEACETYLTRANSFERASE

      I)Perfusion&SectioningProcedure

      1.Perfusethroughtheheartwithafixativesolutioncontaining4%paraformaldehydein0.12Mphosphatebuffer(pH7.3)forlightmicroscopy(LM),andadditionally,0.1%gluteraldehydeand.002%CaCl2forelectronmicroscopy(EM).

      2.Removebrainandpostfix2-18hoursat4°Cin4%paraformaldehydein0.12Mphosphatebuffer.

      3.Afterbrainisblockedforsectioning,washinseveralchangesofbufferfor2-3hours.

      4.SpecimensforEMaresectionedonaVibratome(50μm)andrinsedinbuffer,thoseforLMshouldbecryoprotectedin30%sucroseinbuffer.

      5.Afterfreezingwithdryice,30-40μmthicksectionsofLMspecimensarecutonacryostat.

      6.Sectionsarerinsed,andthenstoredinphosphatebuffercontaining0.1%sodiumazide.

      II)StainingProcedure

      Tissueisprocessedasfreely-floatingsectionsincontinuouslyagitatedsolutions.Allincubationsareperformedatroomtemperatureunlessotherwisestated.

      1.a.ForlocalizingChAT-positivesomataanddendrites:

      Sectionsarewashedin0.1MTris-bufferedsaline(TBS;containing1.4%NaCl,pH7.3)only.Nodetergentorenzymepretreatmentisused.

      b.ForlocalizingChAT-positiveterminal-likestructures:

      IncubatesectionsinTBS(pH8.1)for5minutesat37°C.TransfersectionstoTBS(pH8.1)containingpronase(1.2μg/mL)for11/2-2minutesat37°C,followedbyseveralicecoldbufferwashesforatotalof5minutes.Theconcentrationofpronaseandincubationtimeofthedigestionshouldbeevaluatedforeachregionexamined.

      c.ForlocalizingChATimmunoreactivityandsubsequentlycounterstainingthesections:

      IncubationinTBScontaining0.1%-0.8%TritonX-100for15minutesmayincreasethetissuepenetrationoftheImmunoReagents,butitalsoraisesthebackgroundstaining.

      2.Incubatesectionsinnormalgoatserum(3-5%)foronehour.Theworkingsolutionsofallantiserashouldalsocontainsimilarlydilutednormalgoatserum.

      3.Incubateinanti-ChATmonoclonalantibodysolution(Suggestedworkingdilution1:250,finalworkingdilutionmustbedeterminedbyenduser)for2hoursatroomtemperatureandthenforanadditional6-18hoursat4°C.

      4.Incubatewithsecondantibody(i.e.Goatanti-MouseIgG,Cat.No.:AP124,dilution1:50-100)for1-2hours.

      5.IncubatewithdilutedPAPcomplex(i.e.MousePAP,CatNo.:PAP14,conc.25-50μg/mL)foronehour.

      6.Afterrinsinginbuffer,thesecondantibodyandPAPstepsarerepeatedfor40minutesto1houreachinordertoamplifystainingintensity,particularlyofsmallChAT-containingstructures.

      7.Reactfor15minuteswith0.06%3,3"-diaminobenzidine×4HCl(DAB;dilutedinphosphatebufferedsaline,pH7.3)and0.006%H2O2.

      8.SpecimensforroutineLMarepostfixedfor1minutesin0.005%OsO4(osmiumtetraoxide),andthenmounted,dehydratedandcoverslipped.SelectedregionsblockedforEMarepostfixedin2%OsO4for1hour,enblocstainedwithuranylacetate,andflat-embeddedinEpon-Aralditeresin.
      BIOLOGicalInformation
      ImmunogenCholineacetyltransferasepurifiedfromratbrain.
      Clone1E6
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizescholinergicneuronsinthebrainandspinalcord(CNS).
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Monkey
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryCholinergicsystemsareimplicatedinnumerousneurologicfunctions.AlterationinsomecholinergicneuronsmayaccountforthedisturbancesofAlzheimerdisease.Theproteinencodedbythisgenesynthesizestheneurotransmitteracetylcholine.Alternativesplicevariantshavebeenfoundthatcontainalternative5"untranslatedexons.Threeofthefourdescribedsplicevariantsencodeidentical69kDaproteinswhileonevariantencodesboththe69kDaandalarger82kDaprotein.
      GeneSymbol
      • CHAT
      • ChAT
      • CMS1A2
      • CHOACTase
      • CMS1A
      • EC2.3.1.6
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P28329#CatalyzesthereversIBLesynthesisofacetylcholine(ACh)fromacetylCoAandcholineatcholinergicsynapses.
      SIZE:748aminoacids;82568Da
      DISEASE:SwissProt:P28329#DefectsinCHATarethecauseoffamilialinfantilemyastheniagravis2(FIMG2)[MIM:254210,254200];alsoknownasCMS-EA.FIMG2patientshavemyasthenicsymptomssincebirthorearlyinfancy,negativetestsforanti-AChRantibodies,andabruptepisodiccriseswithincreasedweakness,bulbarparalysis,andapneaprecipitatedbyundueexertion,fever,orexcitement.Inheritanceisautosomalrecessive.
      SIMILARITY:SwissProt:P28329##Belongstothecarnitine/cholineacetyltransferasefamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    蚂蚁淘生物188，秉承蚂蚁淘一贯的严谨态度，由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后，保障正品优质。以“快速好省，为科研提供好产品、好价格”为理念，直接链接原厂家，从全国各地原制造商严格挑选188款科研精品，剔除品牌溢价，188生物新电商，把好的产品带给科研！  力求给你最优质的商品。
  • Q：生物188产品正品保障吗？
    A：生物188质量把控人员具有十年的从业经验，在业界享有良好的口碑；自营商城，直接从厂家采购， 自己的团队负责国际物流和清关，中间没有第三方，所有流程严格把控，100%保证正品，假一罚十。

    Q：下单后可以修改订单吗？
    A：下单后的商品付款之前可以修改；订单付款成功，需要联系我们客服进行修改；客服电话：4000-520-616

    Q：可以开发票吗？
    A：本网站所售商品都是正规清关，均开具16%正规发票，发票金额含配送费金额，另有说明的除外。

    Q：商品几天可以发货？
    A：生物188商品，全部现货销售，付款后即可发货,一般一周内送达！

    Q：如何联系商家？
    A：有任何问题够可以电话咨询我们，全国24小时免费服务热线：4000-520-616 或联系我们的在线客服QQ：1570468124

    Q：收到的商品少了/发错了怎么办？
    A：同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是 部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

    Q：退换货/维修需要多长时间？
    A：一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、 数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

客户服务

在线客服
用户反馈

何为188

极简而严谨，我们仅销售188款生物医学科研用品，款款都是爆款；因为少所以聚焦，聚焦甄选每一款产品，聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知

  • 品质甄选，正品保证

  • 自营电商，厂家直采

  • 极简主义，188精品

常见问题 | 购买相关 | 配送与验收 | 支付问题 | 售后与服务 | 服务协议 | 隐私政策 | 何为188 | 中国智造 | 联系我们
友情链接：

公司版权所有 © 2005-2021 苏州蚂蚁淘生物科技有限公司 苏ICP备17049038号-10