This antibody recognizes GAPDH monomer (36 KDa) and the dimer form.
Form:
Liquid
Isotype:
IgG1
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
Recommend Usage:
ELISA (0.01-0.1 ug/mL)Flow CytometryImmunohistochemistryImmunoprecipitation (2-5 ug/mL)Western Blot (0.1-1 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (Antibody Stabilizer).
Storage Instruction:
Store at 4°C for one month. For long term storage store at -20°C to -80°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Publication Reference
1.
Loss of enhancer of zeste homologue 2 (EZH2) at tumor invasion front is correlated with higher aggressiveness in colorectal cancer cells.Böhm J, Muenzner JK, Caliskan A, Ndreshkjana B, Erlenbach-Wünsch K, Merkel S, Croner R, Rau TT, Geppert CI, Hartmann A, Roehe AV, Schneider-Stock R.J Cancer Res Clin Oncol. 2019 Sep;145(9):2227-2240. doi: 10.1007/s00432-019-02977-1. Epub 2019 Jul 17.
2.
Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping.Munteanu B, von Reitzenstein C, Hansch GM, Meyer B, Hopf C.Anal Bioanal Chem. 2012 Sep 6.
Applications
Western Blot (Tissue lysate)
Tissue lysate from mouse muscle was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 500.
Immunohistochemical staining of human colon tissue stained with GAPDH monoclonal antibody, clone 6C5 (Cat # MAB2549) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
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