Mouse monoclonal antibody raised against native ATP1A1.
Immunogen:
Native purified rabbit ATP1A1.
Host:
Mouse
Reactivity:
Dog, Human, Mouse, Pig, Primates, Rabbit, Rat, Sheep, Xenopus
Specificity:
This antibody is specific to Na, K-ATPase alpha 1 subunit.
Form:
Liquid
Isotype:
IgG1, kappa
Recommend Usage:
Flow Cytometry (1:50-1:200)Immunocytochemistry (1:50-1:200)Immunofluorescence (1:100-1:1000)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200)Western Blot (1:1000-1:10000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.09% sodium azide)
Storage Instruction:
Store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers.Peyrottes I, Navarro V, Ondo-Mendez A, Marcellin D, Bellanger L, Marsault R, Lindenthal S, Ettore F, Darcourt J, Pourcher T.Eur J Endocrinol. 2009 Feb;160(2):215-25. Epub 2008 Nov 24.
2.
Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling.Winterstein C, Trotter J, Kramer-Albers EM.J Cell Sci. 2008 Mar 15;121(Pt 6):834-42. Epub 2008 Feb 26.
3.
ABCG1 influences the brain cholesterol biosynthetic pathway but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo.Burgess BL, Parkinson PF, Racke MM, Hirsch-Reinshagen V, Fan J, Wong C, Stukas S, Theroux L, Chan JY, Donkin J, Wilkinson A, Balik D, Christie B, Poirier J, Lutjohann D, Demattos RB, Wellington CL.J Lipid Res. 2008 Jun;49(6):1254-67. Epub 2008 Feb 26.
4.
Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins.Padilla BE, Cottrell GS, Roosterman D, Pikios S, Muller L, Steinhoff M, Bunnett NW.J Cell Biol. 2007 Dec 3;179(5):981-97. Epub 2007 Nov 26.
5.
Lipid rafts are required for Kit survival and proliferation signals.Jahn T, Leifheit E, Gooch S, Sindhu S, Weinberg K.Blood. 2007 Sep 15;110(6):1739-47. Epub 2007 Jun 6.
Applications
Western Blot (Tissue lysate)
Western blot analysis detecting ATP1A1 in porcine proximal tubule protein, using ATP1A1 monoclonal antibody, clone 464.6 (Cat # MAB2407).
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