This antibody is specific to 3-hydroxykynureinine modifications on N-alpha-acetyl lysine, N-alpha-acetyl histidine, N-alpha-acetyl arginine, and N-alpha-acetyl cysteine. It does not recognize the transaminase enzyme involved in the degradation of tryptophan.
Form:
Liquid
Isotype:
IgG1, kappa
Quality Control Testing:
Antibody Reactive Against 3-OHKYN.
Recommend Usage:
Western Blot (2 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris-glycine, 150 mM NaCl
Storage Instruction:
Store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Publication Reference
1.
3-hydroxykynurenine-mediated modification of human lens proteins: structure determination of a major modification using a monoclonal antibody.Staniszewska MM, Nagaraj RH.J Biol Chem. 2005 Jun 10;280(23):22154-64. Epub 2005 Apr 6.
Applications
Western Blot (Recombinant protein)
Western blot analysis of 3-OHKYN in modified BSA using 3-OHKYN monoclonal antibody, clone P3UI (Cat # MAB2404). 10 second ECL exposure. Lane 1 : BSA (-), Lane 2 : BSA (+).
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