Mouse monoclonal antibody raised against partial recombinant Abca1.
Immunogen:
Recombinant protein corresponding to N-terminus extracellular region of mouse Abca1.
Host:
Mouse
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Isotype:
IgG2b, kappa
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:400)Western Blot (1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis.Wang WH, Hullinger RL, Andrisani OM.J Biol Chem. 2008 Sep 12;283(37):25455-67. Epub 2008 Jul 7.
Applications
Western Blot (Tissue lysate)
Western blot analysis of Abca1 in wild type liver tissue (in RIPA) using Abca1 monoclonal antibody, clone HJ1 (Cat # MAB2326).Lane 1, KO mouse liver tissue.Lane 2, WT mouse liver tissue.
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