Mouse monoclonal antibody raised against native ATP1B1.
Immunogen:
Native purified rabbit ATP1B1.
Host:
Mouse
Reactivity:
Bovine, Dog, Human, Pig, Rabbit, Rat
Specificity:
This antibody is specific to Na, K-ATPase beta 1 subunit.
Form:
Liquid
Isotype:
IgG2a, kappa
Recommend Usage:
Western Blot (1:2500-1:10000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In buffer containing 0.09% sodium azide
Storage Instruction:
Store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling.Winterstein C, Trotter J, Kramer-Albers EM.J Cell Sci. 2008 Mar 15;121(Pt 6):834-42. Epub 2008 Feb 26.
2.
The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated diaphorase activity independent of cytosolic phospholipase A2 regulation.Pessach I, Shmelzer Z, Leto TL, Dinauer MC, Levy R.J Leukoc Biol. 2006 Sep;80(3):630-9. Epub 2006 Jul 14.
3.
Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells.Vagin O, Turdikulova S, Sachs G.J Biol Chem. 2005 Dec 30;280(52):43159-67. Epub 2005 Oct 17.
4.
Use of the H,K-ATPase beta subunit to identify multiple sorting pathways for plasma membrane delivery in polarized cells.Vagin O, Turdikulova S, Yakubov I, Sachs G.J Biol Chem. 2005 Apr 15;280(15):14741-54. Epub 2005 Feb 4.
Applications
Western Blot (Tissue lysate)
Detection of ATP1B1 in rat kidney homogenates (20 ug) using ATP1B1 monoclonal antibody, clone 464.8 (also known as 8A) (Cat # MAB2323).Lane 1, 1 : 2,500 primary antibody.Lane 2, 1 : 5,000 primary antibody.
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