Mouse monoclonal antibody raised against SARS-CoV-2 S.
Immunogen:
SARS-CoV-2 VLP (Virus-like particle).
Host:
Mouse
Reactivity:
Viruses
Form:
Liquid
Purification:
Protein A purification
Purity:
> 98% (SDS-PAGE).
Isotype:
IgG1, kappa
Recommend Usage:
ELISA (1:5000-1:20000)Flow CytometryImmunofluorescence(1:500-1:1000)Western Blot (1:1000-1:5000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4.
Storage Instruction:
Store at 4°C for at least 6 months. For long term storage, store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This monoclonal antibody is derived from SARS-CoV-2 VLP immune mouse.
Datasheet:
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of 293T cells were transfected with SARS-CoV-2 VLP (Virus-like particle) were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution.
Western Blot (Recombinant protein)
Western Blot analysis of different recombinant proteins were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution. Lane 1: SARS-CoV-2 S1-RBD, Lane 2: SARS-CoV-2 S1, Lane 3: SARS-CoV S1-RBD, Lane 4: SARS-CoV S1, and Lane 5: MERS-CoV S1-RBD.
Immunofluorescence
Immunofluorescent staining of 293T cells were transfected with SARS CoV-2 spike plasmid and stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) (1:1000).
Enzyme-linked Immunoabsorbent Assay
ELISA assay of SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) to SARS-CoV-2 S1-RBD, SARS-CoV-2 S1, SARS-CoV S1, SARS-CoV S1-RBD, MERS-CoV S1-RBD and BSA (BSA: negative control).
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