Rabbit monoclonal antibody raised against synthetic phosphopeptide of human AKT1.
Immunogen:
A synthetic phosphopeptide corresponding to residues surrounding S124 of human AKT1.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Immunoprecipitation (1:50)Western Blot (1:500-1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western blot analysis of AKT1 (phospho S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.
Immunoprecipitation
Application Image
Western Blot (Cell lysate)
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Immunoprecipitation
Gene Information
Entrez GeneID:
207
Protein Accession#:
P31749
Gene Name:
AKT1
Gene Alias:
AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
Gene Description:
v-akt murine thymoma viral oncogene homolog 1
Omim ID:
164730, 181500
Gene Ontology:
Hyperlink
Gene Summary:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
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