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Millipore/MAB2013 | Anti-K-Cadherin Antibody, extracellular domain, clone 2B6/MAB2013/100 µg
  • Millipore/MAB2013 | Anti-K-Cadherin Antibody, extracellular domain, clone 2B6/MAB2013/100 µg

Millipore/MAB2013 | Anti-K-Cadherin Antibody, extracellular domain, clone 2B6/MAB2013/100 µg

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货号: MAB2013
品牌: Millipore
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    • Description
      CatalogueNumberMAB2013
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-K-CadherinAntibody,extracellulardomain,clone2B6
      AlternateNames
      • Cadherin-6
      BackgroundInformationCadherinsconstituteafamilyoftransmembraneglycoproteinsinvolvedinCa2+-dependentcell-cellinteractions.Themembersofthisfamilyaredifferentiallyexpressedinvarioustissues.Theyfunctioninthemaintenanceoftissueintegrityandmorphogenesis.Thecadherinsgenerallycontainfiveextracellularrepeats,atransmembranedomainandacytoplasmictailthatbindstothecateninfamilyofcytoskeletalanchoringproteinswhichalsofunctionassignaltransducers.TheextracellulardomainsareresponsIBLeforthespecificityofhomophilicinteractionsbetweencellsexpressingthesamecadherin.CadherinsaredividedintotypeIandtypeIIsubgroups.TypeIcadherinsincludeepithelialcadherin(E-cadherin,cadherin-1oruvomorulin),neuralcadherin(N-cadherinorcadherin-2),placentalcadherin(P-cadherinorcadherin-3)andretinalcadherin(R-cadherinorcadherin-4).Kidneycadherin(K-cadherinorcadherin-6)andosteoblastcadherin(OB-cadherinorcadherin-11)aretypeIIcadherins.Cadherin-6isexpressedintheproximaltubuleepitheliumofthekidneyfromwhichmostrenalcellcarcinomasarederived.
      ProductInformation
      FormatPurified
      PresentationPurifiedimmuoglobulin.LiquidinPBScontaining0.09%sodiumazide.
      StorageandShippingInformation
      StorageConditions1yearat4°Cfromdateofshipment
      Applications
      ApplicationAnti-K-CadherinAntibody,extracellulardomain,clone2B6detectslevelofK-Cadherin&hasbeenpublished&validatedforuseinWB,IC,IH.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunohistochemistry
      ApplicationNotesWesternblot:1:25-1:250
      Immunohistochemistry:1:25-1:50;Frozentissuesectionswithavidin-biotinylatedhorserADIshperoxidasecomplex(ABC)asthedetectionmethod.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.I/
      BIOLOGicalInformation
      ImmunogenAffinitypurifiedextracellulardomainofhumanK-cadherin
      Epitopeextracellulardomain
      Clone2B6
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityReactswiththeextracellulardomainofK-cadherin.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesatypeIIclassicalcadherinfromthecadherinsuperfamily.Theencodedmembraneproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Cadherinsmediatecell-cellbindinginahomophilicmanner,contributingtothesortingofheterogeneouscelltypesandthemaintenanceoforderlystructuressuchasepithelium.Strongtranscriptionalexpressionofthisgenehasbeenobservedinhepatocellularandrenalcarcinomacelllines,suggestingapossibleroleinmetastasisandinvasion.
      GeneSymbol
      • CDH6
      • K-cadherin
      • KCAD
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P55285#Cadherinsarecalciumdependentcelladhesionproteins.Theypreferentiallyinteractwiththemselvesinahomophilicmannerinconnectingcells;cadherinsmaythuscontributetothesortingofheterogeneouscelltypes.
      SIZE:790aminoacids;88309Da
      SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Highlyexpressedinbrain,cerebellum,andkidney.Lung,pancreas,andgastricmucosashowaweakexpression.Alsoexpressedincertainliverandkidneycarcinomas.
      SIMILARITY:SwissProt:P55285##Contains5cadherindomains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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