Rabbit monoclonal antibody raised against synthetic peptide of human CD3D.
Immunogen:
A synthetic peptide corresponding to human CD3D.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Flow Cytometry (1:50)Immunocytochemistry (1:50-1:200)Immunofluorescence (1:50-1:200)Immunohistochemistry (1:50-1:200)Immunoprecipitation (1:50)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate with CD3D monoclonal antibody.
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq
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4000-520-616
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