Rabbit monoclonal antibody raised against synthetic phosphopeptide of human AKT1.
Immunogen:
A synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT1.
Host:
Rabbit
Reactivity:
Human, Mouse
Form:
Liquid
Conjugation:
FITC
Purification:
Protein A/G purification
Isotype:
IgG1, kappa
Recommend Usage:
Flow Cytometry (5 uL/106 cells)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide).
Storage Instruction:
Store at 4°C.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Flow Cytometry
Flow cytometric analysis of U937 cells with AKT1 (phospho S473) monoclonal antibody, clone C7 (FITC) (Cat # MAB18784). Treated with LY294002 (red) or with calyculin A (green).
Application Image
Flow Cytometry
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Gene Information
Entrez GeneID:
207
Gene Name:
AKT1
Gene Alias:
AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
Gene Description:
v-akt murine thymoma viral oncogene homolog 1
Omim ID:
164730, 181500
Gene Ontology:
Hyperlink
Gene Summary:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
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