Mouse monoclonal antibody raised against synthetic 7-Ketocholesterol (7-KC).
Immunogen:
Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Conjugation:
Biotin
Purification:
Protein G purification
Isotype:
IgG2b
Recommend Usage:
ELISA (1:1000)Immunocytochemistry (1:50)Immunofluorescence (1:50)Western Blot (1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction:
Store at -20°C.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of HEK293 with 7-Ketocholesterol monoclonal antibody, clone 7E1 (Biotin) (Cat # MAB16487). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) 7-Ketocholesterol Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.
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