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Millipore/MAB1621 | Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11/MAB1621/300 µL
  • Millipore/MAB1621 | Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11/MAB1621/300 µL

Millipore/MAB1621 | Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11/MAB1621/300 µL

价格: ¥3072.00 市场价: 5120.00

货号: MAB1621
品牌: Millipore
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    • Description
      CatalogueNumberMAB1621
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Neurofilament145kDaAntibody,CT,clone3H11
      BackgroundInformationNeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexsofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.FormoreneurofilamentinformationseeNervousSystemCellTypeSpecificMarkerchartonlineundertheCHEMICONTechnicalSupportsection.
      ProductInformation
      FormatCultureSupernatant
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationAnti-Neurofilament145kDaAntibody,C-terminus,clone3H11detectslevelofNeurofilament145kDa&hasbeenpublished&validatedforuseinWB,IC,IH.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:50-1:1000;freshfrozen,4%PFAfixedtissuesections

      Immunoblotting:1:1000-1:2000ECLdetection.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenRecombinantfusionproteincontainingthelast168aminoacidsofratNF-M.PurifiedfromE.ColiinclusionbodiesbyionexchangechromatographyonDEAE-cellulosein6Murea.
      EpitopeC-terminus
      Clone3H11
      HostMouse
      SpecificityReactswithanepitopedependentonthelast84aminoacidsofratNF-M(145K).ReactsspecificallywithNF-Mfromavarietyofmammalianandavianspecies.Theantibodyisnotsensitivetothephosphorylationstateoftheneurofilament.
      IsotypeIgG1
      SpeciesReactivity
      • Avian
      • Mammals
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • NEFM
      • NF-M
      • NEF3
      • NFM
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P07197#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.
      SIZE:916aminoacids;102448Da
      PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFMisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFMresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction.
      SIMILARITY:SwissProt:P07197##Belongstotheintermediatefilamentfamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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