Mouse monoclonal antibody raised against recombinant human 14-3-3.
Immunogen:
Recombinant protein corresponding to human 14-3-3 from E. coli.
Host:
Mouse
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Ammonium sulfate precipitation
Isotype:
IgG2b, kappa
Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200)Western Blot (1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In HEPES, 150 mM NaCl (50% glycerol, 0.01% BSA, 0.03% sodium azide).
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot
Western Blot analysis of Lane 1: HeLa cell, Lane 2: mouse brain tissue and Lane 3: rat brain tissue lysates with 14-3-3 monoclonal antibody, clone 60C10 (Cat # MAB16206).
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