Mouse monoclonal antibody raised against synthetic peptide of human ACTB.
Immunogen:
A synthetic peptide corresponding to human ACTB.
Sequence:
PKANREKMTQIMFETFNTPAMYVAIQ
Host:
Mouse
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Protein A purification
Isotype:
IgG1
Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)Western Blot (1:500-1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot
Western Blot (Cell lysate) analysis of (1) Human HeLa cell, (2) Human HEK 293 cell, (3) Human A-431 cell, (4) Human HepG2 cell. (5) Mouse NIH-3T3 cell, (6) Rat NBT-II cell.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate shows strong cytoplasmic immunoreactivity in smooth muscle and weak positivity in glandular cells.
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations:
PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
Gene Pathway
Adherens junction
Arrhythmogenic right ventricular cardiomyopathy (ARVC)
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