Mouse monoclonal antibody raised against synthetic peptide of human c-Myc tag.
Immunogen:
A synthetic peptide corresponding to full length human c-Myc tag.
Host:
Mouse
Specificity:
The antibody can detect c-Myc fusion proteins.
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG1
Recommend Usage:
Immunofluorescence (1:1000)Immunoprecipitation (1:200)Western Blot (1:5000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 0.5% BSA, 0.02% sodium azide).
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of 1 ug c-Myc fusion protein. Lane 1: At 1:5000 dilution, Lane 2: At 1:10000 dilution.
Immunofluorescence
Immunofluorescence staining of 293 cells transfected with a c-Myc-tag protein using antibody (Red) and DAPI (Blue).
Immunoprecipitation
Immunoprecipitation analysis of Lane 1: Untransfected 293 cell lysate, Lane 2: Transfected 293 cell lysate with c-Myc-tag fusion protein, Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose), Lane 4: IP (transfected 293 + c-Myc tag monoclonal antibody, clone 3E8 + Protein G agarose), Lane 5: IP (transfected 293 + Protein G agarose), Lane 6: Recombinant protein (E.coli).
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4000-520-616
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