Mouse monoclonal antibody raised against synthetic phosphopeptide of human AKT1.
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT1.
Host:
Mouse
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG1
Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-200)Western Blot (1:1000-2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 0.5% BSA and 0.02% sodium azide).
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of PC-3 cell lysate.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
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4000-520-616
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