Mouse monoclonal antibody raised against partial recombinant human ALK.
Immunogen:
Recombinant protein corresponding to amino acids 1360-1460 of human ALK.
Host:
Mouse
Theoretical MW (kDa):
80, 200
Reactivity:
Human
Form:
Liquid
Purification:
Protein A/G purification
Isotype:
IgG1, kappa
Recommend Usage:
Flow Cytometry (0.5-1 ug/106 cells)Immunofluorescence (0.5-1 ug/mL)Western Blotting (0.5-1 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM PBS.
Storage Instruction:
Store at -20 to -80°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
Download
Publication Reference
1.
Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1.Pulford K, Lamant L, Morris SW, Butler LH, Wood KM, Stroud D, Delsol G, Mason DY.Blood. 1997 Feb 15;89(4):1394-404.
Applications
Western Blot
Western Blot analysis of Lane 1: recombinant protein and Lane 2: HepG2 cell lysate with ALK monoclonal antibody, clone ALK/1503 (Cat # MAB14792).
Immunofluorescence
Flow Cytometry
Application Image
Western Blot
enlarge
Immunofluorescence
Flow Cytometry
Gene Information
Entrez GeneID:
238
Protein Accession#:
Q9UM73
Gene Name:
ALK
Gene Alias:
CD246,Ki-1,TFG/ALK
Gene Description:
anaplastic lymphoma receptor tyrosine kinase
Omim ID:
105590
Gene Ontology:
Hyperlink
Gene Summary:
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3" half of ALK, derived from chromosome 2, is fused to the 5" portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
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